EXPERIMENT NO: 09. ISOLATION OF SENNOSIDES FROM SENNA | T.Y B. PHARM SEM - V PHARMACOGNOSY & PHYTOCHEMISTRY – II

 

T.Y B. PHARM SEM - V

PHARMACOGNOSY & PHYTOCHEMISTRY – II

EXPERIMENT NO: 09

AIM: ISOLATION OF SENNOSIDES FROM SENNA.

REFERENCE:

1.     Dr. Anup K. Chakraborty, Sushma M. Rathod. “Practical in pharmacognosy & phytochemistry – II. Edition 2019, published by S. Vikas & company (Medical publisher), Page no. 41, 42.

2.     S. B. Gokhale, Dr. M.G. Kalaskar, Dr. Yogesh Kulkarni, Dr. S.U Yele. A Practical book of pharmacognosy & phytochemistry – II. First edition, Published by Nirali prakashan. Page No. 21, 22.

REQUIREMENTS:

Apparatus: Electric shaker, Vacuum filter unit, Desiccator, Beaker, Measuring cylinder etc.

Chemicals: Senna leaves/pods powder, Benzene, Ethanol etc.

INTRODUCTION:

SENNA:

Synonym: Indian senna, Alexandrian senna

Biological Source: Sennoside is a glycoside, obtained from the dried leaf lets of Cassia angustifolia & Cassia acutifolia known in commerce as Alexandrin senna & Tinnevelly senna. Family: Leguminose.

Chemical constituents: Senna is an anthraquinone glycoside (O-linkage glycoside). Senna consists of two active Dimeric glycosides, sennoside A and Sennoside B. Both on hydrolyses to give two molecules of glucose and the aglycone sennidin A & B.

Senna also contains Sennoside C and D (Hetero dianthrone).

The other glycosides are palmidin A, Aloe-emodin dianthrone-diglycoside, rhein-anthrone-8-glycoside, rhein-8-diglucoside, aloe-emodin-8-glucoside-emodin-anthrone-diglucoside.

Uses: Purgative, Cathartic, vermifuge.

EXTRACTION PROCEDURE:

1.     Extract 100g powdered leaves with 300 ml benzene for 2 hr on electric shaker, filter in vacuum and distill off the solvent.

2.     The dried marc extract with 300ml 70 % methanol on shaker for 4-6 hrs, filter under vacuum and the marc re-extract with 200 ml of 70 % methanol for 2 hr. filter and combine methanolic extract.

3.     Concentrate methanolic extract and acidify to PH 3.2 by addition of HCI with constant stirring. Set aside the mixture for 2 hr at 5°C. Filter under vacuum and add 1g anhydrous calcium chloride in 13 ml denatured spirit with vigorous stirring.

4.     Adjust PH of the solution to 8 by addition of ammonia solution and set aside for 2 hr. filter the solution by vacuum and dry precipitate over phosphorous pentoxide in a desiccator.

RESULT: The sennoside was isolated from senna leaves powder. The percentage yield of sennosides was found to be……………..