EXPERIMENT NO. 09. DETERMINATION OF ACID INSOLUBLE ASH VALUE OF GIVEN CRUDE DRUG SAMPLE.

 PHARMACOGNOSY AND PHYTOCHEMISTRY – I PRACTICAL

EXPERIMENT NO.09

Aim: Determination of acid-insoluble ash value for a given crude drug sample.

Reference: 1. Mayur R. Bhurat, Md. Rageeb Md. Usman, Sanjay A. Nagdev, Practical Pharmacognosy & Phytochemistry – I, Published by S. Vikas And Company (Medical Publisher) page. No.60.

2. Dr. K.R Khandelwal, Textbook of Practical Pharmacognosy, Published by Nirali Prakashan, Sixth Edition, page. No. 23.9 – 23.10

Apparatus: Forceps, Platinum or Silica crucible with lid, Ash less filter paper, etc.

Chemicals: Crude drug sample, HCI, etc.

Instrument: Muffle Furnace, Digital balance, Desiccator, etc.

Procedure:                                 

Proceed as per the steps mentioned in the procedure for determination of total ash value of a crude drug. Further

(i) Using 25 ml of dilute hydrochloric acid, wash the ash from the dish used for total ash into a 100 ml beaker.

(ii) Place a mere gauze over a Bunsen burner and boil for five minutes.

(iii) Filter through an ‘ashless' filter paper, and wash the residue twice with hot water.

(iv) Ignite a crucible in the flame, cool, and weigh.

(v) Put the filter paper and residue into the crucible; heat gently until vapors cease evolving and then more strongly until all carbon has been removed.

(vi) Cool in a desiccator.

(vii) Weigh the residue and calculate the acid-insoluble ash of the crude drug with reference to the air-dried sample of the crude drug.

 

RESULT: An Acid Insoluble Ash Value of given crude drug sample was calculated and found to be…………….

 

Calculation:  (Write on Journals blank page).

Weight of residue = a g

Y g of the air-dried drug gives 100 x a/y g of acid-insoluble ash Therefore, 100 g of air-dried drugs gives 100 x a/y g of acid-insoluble ash.

Acid insoluble ash of sample = 100x a/y %.