EXPERIMENT NO: 11. TTO STUDY THIN LAYER CHROMATOGRAPHY (TLC) OF CINCHONA ALKALOIDS.T.Y B. PHARM SEM - V PHARMACOGNOSY & PHYTOCHEMISTRY – II

                                                T.Y B. PHARM SEM - V

PHARMACOGNOSY & PHYTOCHEMISTRY – II

EXPERIMENT NO: 11

AIM: To Study Thin Layer Chromatography (TLC) of cinchona alkaloids.

REFERENCE:

1.     Dr. Anup K. Chakraborty, Sushma M. Rathod. “Practical in pharmacognosy & phytochemistry – II. Edition 2019, published by S. Vikas & company (Medical publisher), Page no. 52-53.

REQUIREMENTS:

Apparatus: TLC plates, Glass chamber, Iodine chamber, Hot air oven, Beaker, Capillary etc.

Chemical: Cinchona powder, Chloroform, Methanol, and Ammonia.

PROCEDURE:

Preparation of extract: Powdered sample extracted with methanol. The extract concentrated and residue dissolved in methanol.

Adsorbent (Stationary Phase): Make 15 g silica gel slurry with 20 ml of distilled water. The plates are coated with slurry of silica gel to a width of 0.4mm, allowed to dry at room temperature, activated oven at 120°C for 20 min.

Solvent system (Mobile Phase): The solvent system, Chloroform, Methanol, Ammonia (60:10:01) is poured in the TLC chamber to a depth of 3cm and allow to saturate. The chamber is lined with filter paper on three sides to maintain equilibrium of mobile phase

Application of spots: The above sample applied as two spot on each plate with the help of capillary.

Development of chromatogram: The chromatogram is established by ascending technique, allowing solvent system to reach a height of 10 cm. The plates are withdrawn from the chamber, air-dried dried TLC plate and observe under UV lamp (254nm). The alkaloids shows blue fluorescence. Spray with dragondorff’s reagents and air dry. The reddish brown coloration is observed.  

Rf Value:

The Rf value is calculated by using below formula

RESULT: The TLC of cinchona extract was performed and the Rf value was found to be…………………….