MCQs ON STAINING TECHNIQUE | MICROBIOLOGY MCQs WITH ANSWER KEY

 

MCQ ON STAINING TECHNIQUE

      1.      Which of the following stain is used for negative staining?  A

a.      Nigrosine

b.      Methylene blue

c.      ZNCF

d.     Crystal violet

      2.     In simple staining the color of cocci is  A

a.      Blue

b.      Red

c.      Colourless

d.     Pink

      3.  Which of the following is a differential staining technique?  B

a.      Simple staining

b.      Gram staining

c.      Flagella staining

d.     Spores staining

      4.    In gram staining safranin is used as  B

a.      Primary stain

b.      Counter stain

c.      Decolorizer

d.     Tertiary stain

      5.    In acid fast staining the primary stain is…  B

a.      Crystal violet

b.      Carbol fuchsin

c.      Methylene blue

d.     Nigrosine

      6.    Which of the following organism is acid fast?  A

a.      M.Tuberculosis

b.      E.coli

c.      B.subtilis

d.     P.aeruginosa    

       7.     In acid fast staining the counter stain is…  C

a.      Crystal violet

b.      Carbol fuchsin

c.      Methylene blue

d.     Nigrosine

 8. Staining reagent for gram-positive bacteria is   B

a. Fast green

 b. Crystal violet

c. Haematoxylon

d. Safranin

9. The procedure of Gram staining of bacteria was developed by   D

a. A.D. Gram

b. H.A. Gram

c. A.C. Gram

d. H.C. Gram

10. In Gram-staining, iodine is used as a   B
a) fixative
b) mordant
c) solublizer
d) stain

11. In gram staining technique gram positive bacteria appear as  B

a. Pink

b. Violet

c. both a & b

d. None of these

12.  In gram staining technique gram negative bacteria appear as  A

a. Pink

b. Violet

c. both a & b

d. None of these

13. Which of the following is the example of Gram-negative bacteria?  D
a) Lactobacillus
b) Eschericia coli
c) Staphylococcus aureus
d) Bacillus subtilis

 15. The staining technique used to stain the metachromatic granules of Corynebacte-rium   B

a. Giemsa stain

 b. Alberts stain

c. Acid fast staining

d. Both a and b

16. Acridine dyes are more effective against  A

a. Gram positive

b. Gram negative

c. Ricke Hsia

d. Mycoplasma

17. Metachromatic granules can be stained with B

a. Saffranine

b. Methylene blue

c. Crystal violet

d. Pienic acie

18. Endospores can be stained with  D

a. Safranine

b. Crystal violet

c. Methylene blue

 d. Malachite green

19. The order of stains in Gram-staining procedure is  A

a. Crystal violet, Iodine solution, Alcohol, Saffranine

b. Iodine solution, Crystal Violet, Saffranine, Alcohol

c. Alcohol, Crystal Violet, Iodine solution, Saffranine

d. All of these

20. The action of alcohol during Gram-staining is  C

a. Allows the color

b. It adds color

c. Decolorises the cells

d. None of these

21. Mycobacteria are stained with   D

a. Gram’s staining

b. Simple staining

c. Both a and b

d. Ziehl – Neelsen’s staining

22.  The dye eosinate of methylene blue belongs to which group? C
a) Acidic dye
b) Basic dye
c) Neutral dye
d) Oxazine dye

23.  Which of the following are true for Gram-negative bacteria? D
a) upon alcohol treatment, the permeability of the cell wall increases
b) crystal violet-iodine (CV-I) complex is extracted
c) pore size decreases and the CV-I complex cannot be extracted
d) alcohol treatment increases the permeability of the cell wall and the CV-I complex can be extracted

24. Gram-positive bacteria are usually more susceptible to? C
a) streptomycin
b) tetracyclin
c) penicillin
d) ampicillin

25. Which of the staining technique helps in demonstrating spore structure in bacteria as well as free spores? B
a) Acid-fast stain
b) Endospore stain
c) Capsule stain
d) Flagella stain